منابع مشابه
Towards quantitative in situ hybridization.
In situ hybridization analysis of tissue mRNA concentrations remains to be accepted as a quantitative technique, even though exposure of tissue sections to photographic emulsion is equivalent to Northern blot analysis. Because of the biological importance of in situ quantification of RNA sequences within a morphological context, we evaluated the quantitative aspects of this technique. In calibr...
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As numerical and structural defects in chromosomes are an inevitable consequence of IVF, Pre-implantation genetic diagnosis and screening (PGD/PGS) methods are used for detecting abnormalities in embryos before implantation to the uterus to increase the successful rate of IVF. Pre-implantation genetic diagnosis and screening approaches can be achieved by different techniques such as NGS, CGH an...
متن کاملMultiplexed in situ hybridization using hybridization chain reaction.
CHOI ET AL. l DESCRIBE PROGRAMMABLE IN SITU AMPLIFIERS based on the mechanism of hybridization chain reaction (HCR) that enable simultaneous mapping of multiple target mRNAs within whole-mount zebrafish embryos. In contrast to traditional in situ hybridization approaches, HCR in situ amplification is performed for all target mRNAs simultaneously, so the experimental timeline is independent of t...
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The importance of cancer biomarkers is now increasingly recognized and acted on by regulatory organizations, healthcare providers (from reimbursement through to practicing clinicians), cancer organizations, research institutes, and charities. For example, the American Joint Committee on Cancer (AJCC) states in the opening of its mission statement, “The purpose and scope of the AJCC is to create...
متن کاملChromogenic in situ hybridization: a multicenter study comparing silver in situ hybridization with FISH.
Our purposes were to perform a robust assessment of a new HER2 chromogenic in situ hybridization test and report on concordance of silver in situ hybridization (SISH) data with fluorescence in situ hybridization (FISH) data and on intraobserver and interlaboratory scoring consistency. HER2 results were scored from 45 breast cancers in 7 laboratories using the Ventana (Tucson, AZ) INFORM HER-2 S...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1990
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.23.1